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rabbit polyclonal anti-e-syt1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rabbit polyclonal anti-e-syt1
    (A) The cell lysates of LPS-primed WT and ESyt1−/− BMDMs were incubated with biotin-LPS or LPS-primed WT and ESyt1−/− BMDMs were transfected with Biotin-LPS using electroporation. LPS-binding proteins were pulled down by avidin beads and blotted with anti-Casp-11 and <t>anti-E-Syt1</t> antibodies.
    Rabbit Polyclonal Anti E Syt1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti-e-syt1/pmc10528594-13-0-4
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-e-syt1 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1"

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1

    Journal: Cell reports

    doi: 10.1016/j.celrep.2023.112726

    (A) The cell lysates of LPS-primed WT and ESyt1−/− BMDMs were incubated with biotin-LPS or LPS-primed WT and ESyt1−/− BMDMs were transfected with Biotin-LPS using electroporation. LPS-binding proteins were pulled down by avidin beads and blotted with anti-Casp-11 and anti-E-Syt1 antibodies.
    Figure Legend Snippet: (A) The cell lysates of LPS-primed WT and ESyt1−/− BMDMs were incubated with biotin-LPS or LPS-primed WT and ESyt1−/− BMDMs were transfected with Biotin-LPS using electroporation. LPS-binding proteins were pulled down by avidin beads and blotted with anti-Casp-11 and anti-E-Syt1 antibodies.

    Techniques Used: Incubation, Transfection, Electroporation, Binding Assay, Avidin-Biotin Assay

    (A) Immunoblot analysis of E-Syt1 in cell lysates of WT and ESyt1−/− BMDMs primed with LPS (500 ng/mL, 5 h) and treated with DSS.
    Figure Legend Snippet: (A) Immunoblot analysis of E-Syt1 in cell lysates of WT and ESyt1−/− BMDMs primed with LPS (500 ng/mL, 5 h) and treated with DSS.

    Techniques Used: Western Blot

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Virus, Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Software

    Related Articles

    Incubation:

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1
    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Transfection:

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1
    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Electroporation:

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1
    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Binding Assay:

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1
    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Avidin-Biotin Assay:

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1
    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Western Blot:

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1
    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Virus:

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1
    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Recombinant:

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1
    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1
    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Software:

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1
    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.



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    rabbit polyclonal anti-e-syt1  (Thermo Fisher)
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    Thermo Fisher rabbit polyclonal anti-e-syt1
    (A) The cell lysates of LPS-primed WT and ESyt1−/− BMDMs were incubated with biotin-LPS or LPS-primed WT and ESyt1−/− BMDMs were transfected with Biotin-LPS using electroporation. LPS-binding proteins were pulled down by avidin beads and blotted with anti-Casp-11 and <t>anti-E-Syt1</t> antibodies.
    Rabbit Polyclonal Anti E Syt1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti-e-syt1/pmc10528594-13-0-4
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-e-syt1 - by Bioz Stars, 2026-09
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    Fig. 3: <t>E-Syt1</t> interacts with HSV-1gM in infected cells. HeLa cells were infected 728
    E Syt1 Rabbit Polyclonal, supplied by Bethyl, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) The cell lysates of LPS-primed WT and ESyt1−/− BMDMs were incubated with biotin-LPS or LPS-primed WT and ESyt1−/− BMDMs were transfected with Biotin-LPS using electroporation. LPS-binding proteins were pulled down by avidin beads and blotted with anti-Casp-11 and anti-E-Syt1 antibodies.

    Journal: Cell reports

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1

    doi: 10.1016/j.celrep.2023.112726

    Figure Lengend Snippet: (A) The cell lysates of LPS-primed WT and ESyt1−/− BMDMs were incubated with biotin-LPS or LPS-primed WT and ESyt1−/− BMDMs were transfected with Biotin-LPS using electroporation. LPS-binding proteins were pulled down by avidin beads and blotted with anti-Casp-11 and anti-E-Syt1 antibodies.

    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Techniques: Incubation, Transfection, Electroporation, Binding Assay, Avidin-Biotin Assay

    (A) Immunoblot analysis of E-Syt1 in cell lysates of WT and ESyt1−/− BMDMs primed with LPS (500 ng/mL, 5 h) and treated with DSS.

    Journal: Cell reports

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1

    doi: 10.1016/j.celrep.2023.112726

    Figure Lengend Snippet: (A) Immunoblot analysis of E-Syt1 in cell lysates of WT and ESyt1−/− BMDMs primed with LPS (500 ng/mL, 5 h) and treated with DSS.

    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Techniques: Western Blot

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1

    doi: 10.1016/j.celrep.2023.112726

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

    Techniques: Virus, Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Software

    Fig. 3: E-Syt1 interacts with HSV-1gM in infected cells. HeLa cells were infected 728

    Journal: Journal of Virology

    Article Title: Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion

    doi: 10.1128/jvi.01281-17

    Figure Lengend Snippet: Fig. 3: E-Syt1 interacts with HSV-1gM in infected cells. HeLa cells were infected 728

    Article Snippet: Primary antibodies and dilution used in this study were as follows: anti-336 myc rabbit polyclonal (1:1000; 2272, Cell Signaling Technology), anti-myc mouse 337 polyclonal ( 9B11) (1:100; 2276, Cell Signaling Technology) anti-HA mouse monoclonal 338 (1:1000; SC-7392, Santa Cruz), anti E-Syt1 rabbit polyclonal (1:1000; A303-362A, 339 Bethyl Laboratories), anti E-Syt2 rabbit polyclonal (1:1000; NBP1-59988, Novus 340 Biologicals), anti E-Syt3 rabbit polyclonal (1:1000; NBP1-91354, Novus Biologicals), 341 anti β-actin mouse monoclonal (1:2500; ab6276, Abcam), anti γ-Tubulin mouse 342 monoclonal (1:5000; T6557, Sigma-Aldrich), anti-HSV VP5 mouse monoclonal (1:2000; 343 Virusys), anti-HSV gM rabbit polyclonal PAS980 (1:1000; courtesy of Dr. Lynn 344 Enquist), anti-HSV gM rabbit polyclonal 4c10 (1:000; courtesy of Dr. Joel Baines) and 345 anti-human DDX3 rabbit R648 polyclonal (1:4000; courtesy of Dr. A. Patel).

    Techniques: Infection

    Fig. 5: Impact of the virus on E-Syt1 expression. HeLa cells were mock treated or 750

    Journal: Journal of Virology

    Article Title: Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion

    doi: 10.1128/jvi.01281-17

    Figure Lengend Snippet: Fig. 5: Impact of the virus on E-Syt1 expression. HeLa cells were mock treated or 750

    Article Snippet: Primary antibodies and dilution used in this study were as follows: anti-336 myc rabbit polyclonal (1:1000; 2272, Cell Signaling Technology), anti-myc mouse 337 polyclonal ( 9B11) (1:100; 2276, Cell Signaling Technology) anti-HA mouse monoclonal 338 (1:1000; SC-7392, Santa Cruz), anti E-Syt1 rabbit polyclonal (1:1000; A303-362A, 339 Bethyl Laboratories), anti E-Syt2 rabbit polyclonal (1:1000; NBP1-59988, Novus 340 Biologicals), anti E-Syt3 rabbit polyclonal (1:1000; NBP1-91354, Novus Biologicals), 341 anti β-actin mouse monoclonal (1:2500; ab6276, Abcam), anti γ-Tubulin mouse 342 monoclonal (1:5000; T6557, Sigma-Aldrich), anti-HSV VP5 mouse monoclonal (1:2000; 343 Virusys), anti-HSV gM rabbit polyclonal PAS980 (1:1000; courtesy of Dr. Lynn 344 Enquist), anti-HSV gM rabbit polyclonal 4c10 (1:000; courtesy of Dr. Joel Baines) and 345 anti-human DDX3 rabbit R648 polyclonal (1:4000; courtesy of Dr. A. Patel).

    Techniques: Virus, Expressing

    Fig. 9: E-Syt1 and E-Syt3 down regulation increases cell-to-cell spread. The 798

    Journal: Journal of Virology

    Article Title: Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion

    doi: 10.1128/jvi.01281-17

    Figure Lengend Snippet: Fig. 9: E-Syt1 and E-Syt3 down regulation increases cell-to-cell spread. The 798

    Article Snippet: Primary antibodies and dilution used in this study were as follows: anti-336 myc rabbit polyclonal (1:1000; 2272, Cell Signaling Technology), anti-myc mouse 337 polyclonal ( 9B11) (1:100; 2276, Cell Signaling Technology) anti-HA mouse monoclonal 338 (1:1000; SC-7392, Santa Cruz), anti E-Syt1 rabbit polyclonal (1:1000; A303-362A, 339 Bethyl Laboratories), anti E-Syt2 rabbit polyclonal (1:1000; NBP1-59988, Novus 340 Biologicals), anti E-Syt3 rabbit polyclonal (1:1000; NBP1-91354, Novus Biologicals), 341 anti β-actin mouse monoclonal (1:2500; ab6276, Abcam), anti γ-Tubulin mouse 342 monoclonal (1:5000; T6557, Sigma-Aldrich), anti-HSV VP5 mouse monoclonal (1:2000; 343 Virusys), anti-HSV gM rabbit polyclonal PAS980 (1:1000; courtesy of Dr. Lynn 344 Enquist), anti-HSV gM rabbit polyclonal 4c10 (1:000; courtesy of Dr. Joel Baines) and 345 anti-human DDX3 rabbit R648 polyclonal (1:4000; courtesy of Dr. A. Patel).

    Techniques:

    Fig. 11: Overexpression of E-Syt1 or E-Syt-3 reduces viral yields. HeLa cells 818

    Journal: Journal of Virology

    Article Title: Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion

    doi: 10.1128/jvi.01281-17

    Figure Lengend Snippet: Fig. 11: Overexpression of E-Syt1 or E-Syt-3 reduces viral yields. HeLa cells 818

    Article Snippet: Primary antibodies and dilution used in this study were as follows: anti-336 myc rabbit polyclonal (1:1000; 2272, Cell Signaling Technology), anti-myc mouse 337 polyclonal ( 9B11) (1:100; 2276, Cell Signaling Technology) anti-HA mouse monoclonal 338 (1:1000; SC-7392, Santa Cruz), anti E-Syt1 rabbit polyclonal (1:1000; A303-362A, 339 Bethyl Laboratories), anti E-Syt2 rabbit polyclonal (1:1000; NBP1-59988, Novus 340 Biologicals), anti E-Syt3 rabbit polyclonal (1:1000; NBP1-91354, Novus Biologicals), 341 anti β-actin mouse monoclonal (1:2500; ab6276, Abcam), anti γ-Tubulin mouse 342 monoclonal (1:5000; T6557, Sigma-Aldrich), anti-HSV VP5 mouse monoclonal (1:2000; 343 Virusys), anti-HSV gM rabbit polyclonal PAS980 (1:1000; courtesy of Dr. Lynn 344 Enquist), anti-HSV gM rabbit polyclonal 4c10 (1:000; courtesy of Dr. Joel Baines) and 345 anti-human DDX3 rabbit R648 polyclonal (1:4000; courtesy of Dr. A. Patel).

    Techniques: Over Expression

    Fig. 13: E-Syt1 and E-Syt3 knockdown increase cell-to-cell fusion in infected 843

    Journal: Journal of Virology

    Article Title: Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion

    doi: 10.1128/jvi.01281-17

    Figure Lengend Snippet: Fig. 13: E-Syt1 and E-Syt3 knockdown increase cell-to-cell fusion in infected 843

    Article Snippet: Primary antibodies and dilution used in this study were as follows: anti-336 myc rabbit polyclonal (1:1000; 2272, Cell Signaling Technology), anti-myc mouse 337 polyclonal ( 9B11) (1:100; 2276, Cell Signaling Technology) anti-HA mouse monoclonal 338 (1:1000; SC-7392, Santa Cruz), anti E-Syt1 rabbit polyclonal (1:1000; A303-362A, 339 Bethyl Laboratories), anti E-Syt2 rabbit polyclonal (1:1000; NBP1-59988, Novus 340 Biologicals), anti E-Syt3 rabbit polyclonal (1:1000; NBP1-91354, Novus Biologicals), 341 anti β-actin mouse monoclonal (1:2500; ab6276, Abcam), anti γ-Tubulin mouse 342 monoclonal (1:5000; T6557, Sigma-Aldrich), anti-HSV VP5 mouse monoclonal (1:2000; 343 Virusys), anti-HSV gM rabbit polyclonal PAS980 (1:1000; courtesy of Dr. Lynn 344 Enquist), anti-HSV gM rabbit polyclonal 4c10 (1:000; courtesy of Dr. Joel Baines) and 345 anti-human DDX3 rabbit R648 polyclonal (1:4000; courtesy of Dr. A. Patel).

    Techniques: Knockdown, Infection